Human YWHAG protein, Myc and Fc tags

Catalog Number:

N44Q50

Description:

Two synapse-related proteins, 14-3-3 protein gamma (YWHAG) and Neuronal pentraxin-2 (NPTX2), were found to be the most closely related to measures of cognitive impairment of Alzheimer’s disease (AD). A recombinant form (Met 1 – Asn 247) of human YWHAG protein (Accession: P61981) fused with Myc tag followed by a human Fc (hFc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The purity has been examined by reducing SDS-PAGE, where protein with a calculated MW of ~55 kDa (including tags). We evaluated its binding to a Myc tag-specific antibody (Cat.No. M52T82) in ELISA, see results.

Protein Synonyms:

Protein kinase C inhibitor protein 1 (KCIP-1)

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Met 1 – Asn 247

Tag:

Myc and human Fc (hFc) tags

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.