EpiCD™ CHO
Catalog Number:
K62V37
Description:
EpiCD™ CHO medium is a chemically-defined (CD), free of serum, proteins or growth factors, animal-origin components, and hydrolysates sterile, ready-to-use medium for suspension culture of Chinese hamster ovary (CHO) cell lines to achieve high density growth and robust expression of protein or antibody.
This medium is not compatible with commonly used transfection reagents, therefore, cell medium should be changed to EpiCD™ CHO Expression medium (Cat. No. M13U59) before the transfection.
EpiCD™ CHO Feed (Cat. No.M29V67) is designed for use with this medium to extend cell survival time and improve protein quality and metabolite profile.
Applications:
This medium supports multiple applications including:
- Support transfected CHO suspension culture: CHO-K1, CHO-DG44, CHO-S and CHO-K1SV in multiple perfusion processes: intensified fed-batch, concentrated fed-batch, and continuous perfusion.
- Not Compatible with commonly used transfection reagents.
- Scalable production of recombinant protein or antibody to gram-scale yields.
- Maximum batch culture cell densities and protein titers are needed.
Specifications:
| Appearance (liquid) | Orange red clear liquid |
| pH | 6.9-7.6 |
| Osmolality（mOsm/kg） | 270-330 |
| Storage | 2 – 8℃, dark and dry |
Culture Conditions:
- Cell medium: EpiCD™ CHO medium, sterile liquid, doesn’t contain HT or L-glutamine. L-glutamine and Anti-Clumping Agent can be added per user.
- Cell line: CHO based cell lines for suspension cultures.
- Culture type: Suspension culture.
- Culture container: shake flasks, TPPs, bioreactors, etc.
- Temperature range: 37℃±5, temperature shift as needed for cell culture conditions.
- Shaker incubator setting: 5 – 8% CO2, ≥ 80% humidity, shaker speed 110-150rpm (amplitude: 50mm)
Ordering:
- K62V37-1L for 1 liter liquid.
- K62V37-10L for 10 liter powder. Easy reconstitution without additives required. See below steps.
- Larger pack sizes available upon request.
Powder Reconstitution (briefly)
- Use 24 g/L of ultrapure water with low ion concentration or WFI.
- Mix for a minimum of 40 min by stirring.
- Pass 0.22µm filter. Ready to use.
Product Statement:
For Further Manufacturing or Research Use. Not For Diagnostic or Therapeutic Use.
Legal Information:
EpiCD is a trademark of ReVacc Inc, USA
Free Sample:
Free Sample is available per request, fill this form
