Human Beta-amyloid protein, Myc and Fc tags

Catalog Number:

X77D63

Description:

The cognitive decline in people with Alzheimer’s disease (AD) involves an abnormal buildup of the proteins Amyloid beta (Aβ) and tau. A recombinant form (Leu 18 – Gly 700) of human beta-amyloid precursor A4 protein (Accession: P05067) fused with Myc tag followed by a human Fc (hFc) tag at the C-terminus was produced by human embryonic kidney HEK cells, followed by purification. The purity has been examined >95% as determined by reducing SDS-PAGE, where protein with a calculated MW of ~107 kDa (including tags). We evaluated its binding to a Myc tag-specific antibody (Cat.No. M52T82) and Amyloid beta-specific antibody (clone RV91, Cat.No. V74R16) in ELISAs, see results.

Protein Synonyms:

ABPP, APP, APPI, Amyloid-beta A4 protein, Alzheimer disease amyloid A4 protein homolog

Applications:

Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB), Immunization or Antibody Sorting Probe, and other biological assays

Source:

Human embryonic kidney HEK cells

Species:

Human

Sequence:

Leu 18 – Gly 700

Tag:

Myc and human Fc (hFc) tags

Size and Formulation:

100 or 500 μg in PBS, pH7.2, without preservatives.

Note: Not lyophilized formulation, so no lyophilized protectant reagents added.

Endotoxin:

Less than 1.0 EU per μg by the LAL method / rFC method.

Shipping or storage condition

Ship with dry ice. Recommend -80°C storage for long-term. Store at 4°C or −20 °C for short-term.  Multiple freeze/Thaw cycles will reduce its activity. Aliquot is recommended.

Note:

For research use only.